Our Core Technology

ImmunoScape unlocks high-impact visibility into the immune system

Our proprietary multiplexed tetramer technology surpasses the limitations of flow cytometry and mass cytometry (CyTOF) for broader and deeper profiling of antigen-specific T-cells. Deep multiplexing is made possible by optimizing tetramers for high avidity TCR-antigen staining with combinatorial coding algorithms to produce unique metal barcodes. In a single sample, up to 120 antigen specificities can be interrogated, with several hundreds of parameters possible.

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TargetScape® Single Cell Assay

A powerful new method for revealing phenotype and antigen specificity at unprecedented resolution.

Our TargetScape® signature assay delivers deep immune profiling of antigen-specific CD8+ T cells utilizing a novel barcoding MHC-tetramer method for reliable and highly sensitive analysis of unstimulated blood or dissociated tissue. Reveal the true identity of rare T cell specificities in their native state while acquiring millions of cells in the same sample and building confidence in results.

TargetScape® helps deliver novel insights in studies of immune checkpoint blockade, neoantigen detection, discovery of biomarker signatures, therapeutic and prophylactic vaccine development, T cell therapies, and other areas.

 

Key Benefits

  • Accelerate epitope screening for potential discovery of neoantigens and biomarker signatures
  • Overcome challenges of typical workflows in the detection of rare event antigen-specific T cell responses delivering high quality data you can count on
  • Correlate longitudinal antigen-specific CD8+ T cell responses and associated phenotypes with clinical outcomes to discover novel immune response patterns

References

White Paper

Webinar

UltraScape® Single Cell Assay

Unravel new frontiers in Immune Profiling

The UltraScape® assay is designed to deeply characterize and assess the modulation of multiple key immune cell populations longitudinally, assessing the immune landscape with respect to cellular activation, proliferation, differentiation, trafficking, apoptosis, and inhibitory molecule expression, simultaneously. In addition, it can be seamlessly integrated with our TargetScape® antigen-specific CD8+ T cell discovery assay to achieve even deeper immunological insights.

Key Benefits

  • Extensively profile multiple immune cell subsets (such as T, B, NK, and monocytes) on a single cell level using 120 carefully selected antibodies in one sample
  • Correlate longitudinal immune responses and associated phenotypes with clinical outcomes to to discover novel immune response patterns
  • Obtain deeper value-added information from the same precious sample while preserving additional aliquots for complimentary assays
  • Performed with unstimulated samples direct ex vivo, to obtain a ‘snapshot’ of the in vivo context

 

Cytographer® Bioinformatics Tool – Secure Cloud-based Analysis for Breakthrough Immune Profiling

Designed by Immunologists for Immunologists, previously only available to few leading academic institutions

Cytographer® is ImmunoScape’s analytical platform for high-dimensional immune profiling data. It comprehensively analyses data from our industry leading TargetScape® and UltraScape® single cell immune profiling assays with a highly intuitive user interface that was designed by immunologists who understand best how to overcome the time-consuming challenges of traditional high-dimensional single cell data analysis.

Together with our team, we offer expert analytical modelling and immunological data interpretation. Data generated through ImmunoScape’s immune profiling partnerships are securely stored and hosted by Amazon Web Services (AWS). Custom analysis may be designed based on your individual biological question. You may also request access to Cytographer® for further independent analysis.

Key Features and Benefits:

  • Identify confidently antigen-specific T cells reliably with Cellular Subset ID using advanced automatic clustering and immune subset detection methods (Phenograph, FlowSOM)
  • Unravel differences between cell subsets through ImmunoScape’s breakthrough statistical analysis algorithms
  • Generate insight with high-dimensional data reduction and data visualization using built-in conventional (heatmaps, principal component analysis) and high-dimensional methods (t-SNE, UMAP, Isomap, Diffmap, OneSENSE, PHATE)
  • Benefit from algorithms specifically designed for statistical correlation to clinical data using classical statistics and automated method pipelines
  • Enhance laboratory efficiency with high-throughput data processing using multiplex analysis strategy generation/deconvolution, manual or automatic algorithm-based gating

Whitepaper

Cytographer Work Flow

 

 

TCR antigen profiling (TAP) to assess the antigen-specificity and repertoire

The initiative to establish TCR repertoire analysis in immunoSCAPE opens up new possibilities and the benefits are two fold (i) As a powerful tool,  it will allow us to obtain critical insights into the complexity of TCR repertoire of antigen-specific CD8+ T cells which will inform the design of future diagnostics strategy (ii) will add value to the existing resources at our disposal and provide immunoSCAPE with unique capability of extending these assays to tackle new biological problems.

TCR antigen profiling (TAP)

Our specific interest is to characterize the TCR repertoire of antigen-specific CD8+ T cells. Despite the advancements in studying cellular diversity and function, identification of target antigens remains a challenge. TCR antigen profiling (TAP) is a tool designed to address such important and challenging questions. At the heart of this initiative is the building of capacity to screen and detect antigen-specific CD8+ T cells and uncover their TCR repertoire. TAP technology will help us to maximize the utilization of precious clinical samples to obtain unique information.

At ImmunoSCAPE we have an established workflow for identifying T cell receptor and their cognate antigens by combining FACSorting and single cell sequencing capabilities.  Briefly CD8+ T cells are stained with oligo tagged MHC Class I and a panel of oligo tagged antibodies that are specific to cell surface proteins of interest. Antigen specific T cells are enriched by FACsorting. Subsequently the enriched T cells are subjected to single cell sequencing to obtain TCR, antigen and cell surface antibody specific readouts.

Data analysis pipeline:

  • Single cell sequencing data analysis
    • Extract barcodes & UMIs, Trim reads, De novo assembly & annotate, filter, count UMIs call cells, clonotypes (Cell Ranger)
  • Statistical analysis
    • (Custom R scripts, Excel)
  • High-dimensional data reduction and data visualization
    • Conventional (heatmaps, principal component analysis) and high-dimensional methods (t-SNE, UMAP)
  • Cytographer- Overlaying single cell sequencing and CyTOF datasets from earlier studies at ImmunoScape
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