High-dimensional immune cell profiling technologies have become indispensable for creating maps that help navigate how immunotherapy affect the immune response in cancer, autoimmune, and infectious diseases.
immunoSCAPE leverages flow and mass cytometry platforms for in-depth characterization of immune cell subsets.
Mass cytometry or Cytometry by Time-Of-Flight (CyTOF®, Fluidigm) allows simultaneous assessment of more than 40 parameters in a single sample. This is achieved by tagging of antibodies or other probes with metal isotopes, which are then detected at a single cell level in a mass cytometer.
High-dimensional immune profiling can identify a large number of different immune cell subsets within a blood or tissue sample, or broadly and finely characterize phenotype and function of target immune cell populations, such as T cells, NK cells and other innate lymphoid cells, B cells, monocyte/macrophages or dendritic cells.
immunoSCAPE is a leading expert in antibody panel design and downstream analysis and interpretation of complex immunological data. We have innovative methods for in-depth characterization of multiple immune subsets within a single sample.
We routinely design and validate high-dimensional phenotypic and functional panels for in-depth characterization of mouse and human immune cell subsets in blood and tissues.
Antibody panel design is challenging – it requires:
Immunological understanding to match relevant cellular markers to specific biological questions
Validation of antibody-metal pairs that will give robust specific mass cytometry signal on the expected cell population
Assembly of tagged-antibody combinations which maintain good signal discrimination for all markers without any interference
Immunomonitoring and Biomarker Discovery
Our technology measures expression of both surface and intracellular or intranuclear markers. Detection of transcription factors, proliferation markers, effector molecules such as perforin or cytokines allows to gain deeper insights in the functional capability of cells.
Immune profile maps and data are then used to assess alterations of the immune response driven by disease or treatment, or associated with clinical response for robust immunomonitoring.
Application examples can be found in publications.